my C.V.
Guo-Zhong
EDUCATION
Fudan University, Institutes of Brain Science, State Key Laboratory of Medical neurobiology Shanghai, CHINA
Ph.D. in neurobiology Sept. 2012 ~ Oct. 2017
Advisor: Prof. XXX, Prof. XXX
Title of dissertation: “An optogenetic study of non-glutamatergic excitatory inputs to mouse retinal ganglion cells”.
Changchun University of Science and Technology, School of Photo-electronic Engineering Changchun, CHINA
Master in optical engineering, Sept. 2008 ~ Apr. 2011
Changchun University of Science and Technology, School of Life Science and Technology Changchun, CHINA
Bachelor’s in biomedical engineering, Sept. 1999 ~ Jul. 2003
RESEARCH EXPERIENCE
1. project “Optogenetic dissection of inhibitory neural circuits in the inner retina”
I examined 7 transgene mouse lines’ specificity by immunohistochemistry. Finally, I picked up VGAT-ChR2-EYFP mouse as the model animal for analyzing the inner retina inhibitory neural circuits and further identified the transgene Channelrhodopsin-2’s functionality by patch clamp and optogenetics. By chance, I found it is common to see ganglion cells having not only IPSC, but also EPSC, in the condition of blocking glutamatergic transmission, even blocking ACh transmission, when a population of amacrine cells was optogenetically activated. These results propose a possibility of the existence of an unknown co-transmission system in the retina amacrine cells. We have published a part of this work in Neuroscience in November 2017 (see also publication list [2]), and the remaining work is ongoing by my labmate.
2. Project “the mechanism of orexin modulating excitatory and inhibitory transmission in the retina ganglion cells”
I investigated the expression of orexins and their receptors in rat retina, localized the orexin A/B and its receptor1/2 in the inner retina circuits with immunofluorescence labelling techniques. This work opened a serial research projects about orexin in the retina in our lab. See also publishing list [4,1].