It differs according to the word, But generally it doesn't affect the meaning and any one read a word he will understand what you mean easily, Just try to stick to the grammers as long as you can

as I know In the meaning the same and what you are asking about called hamaz wasel ا and hamaza qat3 أ and to know different put letter wow و before word and check if you pronoun hamaza so it is qat3 أ if not wasel ا am not expert in this field so telling you what I know

هي لا يتغير المعنى

أ _هذه تسمى همزة القطع ا _ وهذه تسمى همزة الوصل همزة القطع تكتب في بعض الكلمات مثلا إبتسامه ، إنسان ، وهمزة الوصل تكتب في بعض الكلمات مثلا : القمر ، الشمس ، استغفار ،

An easy example to understand the diffrence .. بَعدَ أذُنِكَ after your ear بَعْدَ إذْنِكَ after your permission but if you have this situation بعد اذنك you can't even a native can't distinguish if it's ear or permission only if it's clear in the context .. but if you find it like بعد أذنك it's 99% refering to ear this is why it's wrong to write it like اذنك So the Summary and the result , yep , there is a diffrence between أذنك and اذنك but don't complain a lot about it , it will not create a big problem after all :)