There are a lot of meanings for each! Here are the most common uses and some examples. IN: Being physically inside a location or space: "The book is in the box." "I am in the building" Being inside something abstract: "I can't explain it in English." "It is hot in the summer." Within a certain period of time: "I will go there in ten minutes." Other words that are like IN are WITHIN, INSIDE, or INTO ON: On top of (encima de) "Put the blanket on the baby" "There are leaves on the ground." Where something is located "I have dirt on my face" "My house is on this street" How something is conveyed "I'm talking on the phone" "The game is on TV" An occurrence (usually a day of the week or a date) "We went on Sunday" "The party is on June 14" An appliance that is being used "The lights are on." "I turned the TV on" Other words/phrases that are like ON are ON TOP OF, UPON AT: Specific location "I am at the park." "I am at school." A certain time of an occurrence "The party is at 1:00." "We read at night." Direction "She is looking at me." "I throw the ball at the target." I hope this helps!

Sí, hay diferencias.