Effect of Black Seed on Protein Band Profiles of Blood Serum And Spleen Histopathological Findings Free radicals in cigarette smoke can cause systemic inflammation and induce oxidative stress that affects the spleen as secondary lymphoid organs and the production of stress proteins in blood serum. Free radical activity can be inhibited by antioxidants contained in black seed (Nigella sativa). The purpose of this study was to determine the effect of black cumin extract on protein band profiles of the serum and spleen histopathology in 21 days nonfilter-tipped cigarette smoke exposed rats. An experimental study using 20 male rats (Rattus norvegicus) strain Wistar were divided into 5 groups, as follows: the negative control group, the positive control group, the first, the second, and the third treatment groups (exposed to nonfilter-tipped cigarette smoke and treated with black seed extract 0.6; 1.2; 2.4 g / kg / day subsequently). Protein band profiles were measured using electrophoresis techniques. Rat spleen histopathology preparations made using Haematoxcylin eosin (HE) staining to observe changes in histological structure.